ABSTRACT
Allah Al-Mighty creates diverse flora and fruits, as mentioned in QS. al - An’am: 99, QS.al-Mukminun:19, QS. ar-Ra’d: 4 and QS. an- Nahl: 11. All of Allah’s creation are for human being welfare as food, drug and others. Prabu Betari Grape is one of the species from Vitaceae that contains anthocyanin compound. Prabu Betari Grape is known to contain anthocyanin which has several pharmacology activities, such as antioxidant activities and anticancer. The aim of this research to isolate and identification anthocyanin from grape skin. Grape skin crude material was macerated by variation of solvent (metanol p.a, etanol 95% and water containing 1% hydrochloride acid). Extracted by thin layer chromatography using silica gel F254 as stationary phase and variation eluen there are Forestal= HCl-acetic acid-water (3:30:10), n-buthanolacetic acid glacial-water (4:1:5), Format=HCl-formic acid (2:5:3), ethyl aceticacetic acid glacial-formic acid-water (10:1,1:1,1:2,6), and n-buthanol-acetic acid glacial-water (5:1:1) as mobile phase.